冷诱导RNA结合蛋白通过激活NF-κB信号通路影响H1N1甲型流感病毒的复制

doi:10.11843/j.issn.0366-6964.2016.10.020

Abstract

Abstract:

The cold-inducible RNA-binding protein (CIRP) is an upstream regulator of the NF-κB and ERK pathways，which are essential for the replication of the influenza virus and the initial immune response.In order to investigate the effect of CIRP on the replication of H1N1 influenza A virus and its possible molecular mechanism，in this study，CIRP overexpression BHK-21 (Cirp+BHK-21) cells were constructed，and the phosphorylation levels of NF-κB and ERK in Cirp+BHK-21 cells were detected by Western blot to confirm the effect of CIRP on regulation of NF-κB and ERK1/2；Real-time RT-PCR was used to detect the dynamic changes of virus load in Cirp+BHK-21 and control cells after infected with influenza A virus，the method was also used to detect the dynamic changes of virus load in Cirp+BHK-21 cells which were blocked by the NF-κB inhibitor PDTC.The results of Western blot exhibited that overexpressed CIRP could significantly increase the expression of phosphorylation level of NF-κB (P<0.05)，but had no significant effect on the phosphorylation level of ERK.The results of quantitative detection of virus showed that overexpressed CIRP could significantly enhance the proliferation of influenza A virus，the virus load in the Cirp+BHK-21 cells were 111%，103%，167% and 235% (P<0.05) at 3，9，15 and 21 h PI，respectively，compared to the control group；Blocking the NF-κB was significantly decreased the virus load in the Cirp+BHK-21，and the virus load in treatment group were 98%，42%，19% (P<0.05)，7% (P<0.05) at 3，9，15 and 21 h PI，respectively，compared to the unblock group.Therefore，this study confirmed that overexpressed CIRP could enhance the proliferation of influenza A virus via activation of NF-κB pathway.

CLC Number:

S852.23

NIE Pei-ting，TANG Cheng，YUE Hua. Activation of Cold-inducible RNA-binding Protein by H1N1 Influenza Virus Contributes to Viral Replication Via Activating NF-κB Pathway[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(10): 2108-2114.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2016.10.020

https://www.xmsyxb.com/EN/Y2016/V47/I10/2108

References

［1］殷震，刘景华.动物病毒学［M］.2版．北京：科学出版社，1997：704-712.
YIN Z，LIU J H.Animal virology［M］.Beijing：Science Press，1997：704-712.(in Chinese)
［2］EMARA M M，BRINTON M A.Interaction of TIA-1/TIAR with West Nile and dengue virus products in infected cells interferes with stress granule formation and processing body assembly［J］.Proc Natl Acad Sci U S A，2007，104(21)：9041-9046.
［3］COOMBS K M，BERARD A，XU W，et al.Quantitative proteomic analyses of influenza virus-infected cultured human lung cells［J］.J Virol，2010，84(20)：10888-10906.
［4］KÖNIG R，STERTZ S，ZHOU Y，et al.Human host factors required for influenza virus replication［J］.Nature，2010，463(7282)：813-817.
［5］YANG X X，DU N，ZHOU J F，et al.Gene expression profiles comparison between 2009 pandemic and seasonal H1N1 influenza viruses in A549 cells［J］.Biomed Environ Sci，2010，23(4)：259-266.
［6］CHAKRABARTI A K，VIPAT V C，MUKHERJEE S，et al.Host gene expression profiling in influenza A virus-infected lung epithelial (A594)cells：a comparative analysis between highly pathogenic and modified H5N1 viruses［J］.Virol J，2010，7：219.
［7］LEE S M，GARDY J L，CHEUNG C Y，et al.Systems-level comparison of host-responses elicited by avian H5N1 and seasonal H1N1 influenza viruses in primary human macrophages［J］.PLoS One，2009，4(12)：e8072.
［8］BRASS A L，DYKXHOORN D M，BENITA Y，et al.Identification of host proteins required for HIV infection through a functional genomic screen［J］.Science，2008，319(5865)：921-926.
［9］KARLAS A，MACHUY N，SHIN Y，et al.Genome-wide RNAi screen identifies human host factors crucial for influenza virus replication［J］.Nature，2010，463(7282)：818-822.
［10］SHAPIRA S D，GAT-VIKS I，SHUM BO，et al.A physical and regulatory map of host-influenza interactions reveals pathways in H1N1 infection［J］.Cell，2009，139(7)：1255-1267.
［11］DENG T，ENGELHARDT O G，THOMAS B，et al.Role of ran binding protein 5 in nuclear import and assembly of the influenza virus RNA polymerase complex［J］.J Virol， 2006，80(24)：11911-11919.
［12］ENGELHARDT O G，SMITH M，FODOR E.Association of the influenza A virus RNA-dependent RNA polymerase with cellular RNA polymerase II［J］.J Virol，2005，79(9)：5812-5818.
［13］HUARTE M，SANZ-EZQUERRO J J，RONCAL F，et al.PA subunit from influenza virus polymerase complex interacts with a cellular protein with homology to a family of transcriptional activators［J］.J Virol，2001，75(18)：8597-8604.
［14］JORBA N，JUAREZ S，TORREIRA E，et al.Analysis of the interaction of influenza virus polymerase complex with human cell factors［J］.Proteomics，2008，8(10)：2077-2088.
［15］KAWAGUCHI A，NAGATA K.De novo replication of the influenza virus RNA genome is regulated by DNA replicative helicase，MCM［J］. EMBO J，2007，26(21)：4566-4575.
［16］WATANABE T，WATANABE S，KAWAOKA Y.Cellular networks involved in the influenza virus life cycle［J］.Cell Host Microbe，2010，7(6)：427-439.
［17］PARK YW，WILUSZ J，KATZE MG.Regulation of eukaryotic protein synthesis：selective influenza viral mRNA translation is mediated by the cellular RNA-binding protein GRSF-1［J］.Proc Natl Acad Sci U S A，1999，96(12)：6694-6699.
［18］KASH J C，CUNNINGHAM D M，SMIT M W，et al.Selective translation of eukaryotic mRNAs：functional molecular analysis of GRSF-1，a positive regulator of influenza virus protein synthesis［J］.J Virol，2002，76(20)：10417-10426.
［19］GOODMAN A G，SMITH J A，BALACHANDRAN S，et al.The cellular protein P58IPK regulates influenza virus mRNA translation and replication through a PKR-mediated mechanism［J］.J Virol，2007，81(5)：2221-2230.
［20］PREDICALA R，ZHOU Y.The role of ran-binding protein 3 during influenza A virus replication［J］.J Gen Virol，2013，94(Pt 5)：977-984.
［21］NISHIYAMA H，ITOH K，KANEKO Y，et al.A glycine-rich RNA-binding protein mediating cold-inducible suppression of mammalian cell growth［J］.J Cell Biol，1997，137(4)：899-908.
［22］XUE J H，NONOGUCHI K，FUKUMOTO M，et al.Effects of ischemia and H₂O₂ on the cold stress protein CIRP expression in rat neuronal cells［J］.Free Radie Biol Med，1999，27(11-12)：1238-1244.
［23］WELLMANN S，BÜHRER C，MODEREGGER E，et al.Oxygen-regulated expression of the RNA-binding proteins RBM3 and CIRP by a HIF-1-independent mechanism［J］.J Cell Sci，2004，117(Pt 9)：1785-1794.
［24］PAN F，ZARATE J，CHOUDHURY A，et al.Osmotic stress of salmon stimulates upregulation of a cold inducible RNA binding protein (CIRP) similar to that mammals and amphibians［J］.Biochimine，2004，86(7)：451-461.
［25］YANG C，CARRIER F.The UV-inducible RNA-binding protein A18（A18 hnRNP）plays a protective role in the genotoxic stress response［J］.J Biol Chem，2001，276(50)：47277-47284.
［26］DE LEEUW F，ZHANG T，WAUQUIER C，et al.The cold-inducible RNA-binding protein migrates from the nucleus to cytoplasmic stress granules by a methylation-dependent mechanism and acts as a translational repressor［J］.Exp Cell Res，2007，313(20)：4130-4144.
［27］SAITO K，FUKUDA N，MATSUMOTO T，et a1.Moderate low temperature preserves the stemness of neural stem cells and suppresses apoptosis of the cells via activation of the cold-inducible RNA binding protein［J］. Brain Res，2010，1358：20-29．
［28］LAN D，TANG C，LI M，et al.Screening and identification of differentially expressed genes from chickens infected with Newcastle disease virus by suppression subtractive hybridization［J］.Avian Pathol，2010，39(3)：151-159.
［29］周鸿淼，汤承，岳华，等.冷诱导RNA结合蛋白参与小鼠对LPS的应答［J］.畜牧兽医学报，2014，45（8）：1348-1354.
ZHOU H M，TANG C，YUE H，et al.Cold-inducible RNA binding protein involves in response to LPS in mice［J］.Acta Veterinaria et Zootechnica Sinica，2014，45(8)：1348-1354.(in Chinese)
［30］ARTERO-CASTRO A，CALLEJAS F B，CASTELLVI J，et al.Cold-inducible RNA-binding protein bypasses replicative senescence in primary cells through extracellular signal-regulated kinase 1 and 2 activation［J］.Mol Cell Biol，2009，29(7)：1855-1868.
［31］PLESCHKA S，WOLFF T，EHRHARDT C，et al.Influenza virus propagation is impaired by inhibition of the Raf/MEK/ERK signalling cascade［J］.Nat Cell Biol，2001，3(3)：301-305
［32］LUDWIG S，PLANZ O.Influenza viruses and the NF-κB signaling pathway-towards a novel concept of antiviral therapy［J］.Biol Chem，2008，389(10)：1307-1312.
［33］SCHMOLKE M，VIEMANN D，ROTH J，et al.Essential impact of NF-kappaB signaling on the H5N1 influenza A virus-induced transcriptome［J］.J Immunol，2009，183(8)：5180-5189.
［34］NIMMERJAHN F，DUDZIAK D，DIRMEIER U, et al.Active NF-кB signalling is a prerequisite for influenza virus infection［J］.J Gen Virol，2004，85(Pt 8)：2347-2356.
［35］TANG C，WANG Y，LAN D，et al.Analysis of gene expression profiles reveals the regulatory network of cold-inducible RNA-binding protein mediating the growth of BHK-21 cells［J］.Cell Biol Int，2015，39(6)：678-689.
［36］聂培婷，汤承，岳华.H1N1甲型流感病毒在BHK21中的增殖规律［J］.西南民族大学学报，2012，38(5)：764-769.
NIE P T，TANG C，YUE H.Study on the proliferation profile of influenza A virus in BHK21 cells［J］.Journal of Southwest University for Nationalities，2012，38(5)：764-769.(in Chinese)
［37］吴巧，张斌，汤承，等.H5N1禽流感病毒感染小鼠后内参基因的筛选［J］.中国畜牧兽医，2013，40(9)：55-60.
WU Q，ZHANG B，TANG C，et al.Selection of reference gene in mice infected with H5N1 avian influenza virus［J］.China Animal Husbandry & Veterinary Medicine，2013，40(9)：55-60.(in Chinese)
［38］MOGENSEN T H，MELCHJORSEN J，HÖLLSBERG P，et al.Activation of NF-κB in virus-infected macrophages is dependent on mitochondrial oxidative stress and intracellular calcium：downstream involvement of the kinases TGF-beta-activated kinase 1，mitogen-activated kinase/extracellular signal-regulated kinase kinase 1，and l kappa B kinase［J］.J Immunol，2003，170(12)：6224-6233.
［39］WURZER W J，EHRHARDT C，PLESCHKA S，et al.NF-кB-dependent induction of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and Fas/FasL is crucial for efficient influenza virus propagation［J］.J Biol Chem，2004，279(30)：30931- 30937.
［40］WURZER W J，PLANZ O，EHRHARDT C，et al.Caspase 3 activation is essential for efficient influenza virus propagation［J］. EMBO J，2003，22(11)：2717-2728
［41］FALEIRO L，LAZEBNIK Y.Caspases disrupt the nuclear-cytoplasmic barrier［J］.J Cell Biol ，2000，151(5)：951-959.
［42］KRAMER A，LIASHKOVICH I，OBERLEITHNER H，et al.Apoptosis leads to a degradation of vital components of active nuclear transport and a dissociation of the nuclear lamina［J］.Proc Natl Acad Sci U S A，2008，105(32)：11236-11241.
［43］PAULI E K，SCHMOLKE M，WOLFF T，et al.Influenza A virus inhibits type I IFN signaling via NF-κB-dependent induction of SOCS-3 expression［J］.PLoS Pathog，2008，4(11)：e1000196.
［44］WEI L，SANDBULTE M R，THOMAS P G，et al.NF-кB negatively regulates interferon-induced gene expression and anti-influenza activity［J］.J Biol Chem，2006，281(17)：11678- 11684.
［45］KUMAR N，XIN Z T，LIANG Y，et al.NF-κB signaling differentially regulates influenza virus RNA synthesis［J］.J Virol，2008，82(20)：9880- 9889.

[1]	JIN Meilin, LI Taotao, SUN Dongxiao, WEI Caihong. Research Progress of Epigenetic Regulation in Fat Deposition Mechanism of Livestock and Poultry [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 855-867.
[2]	LIU Ying, WANG Huanhuan, YAN Shuping, ZHU Bin, ZHANG Yuanshu. Study on the Interaction of Two Pathways of Renin Angiotensin System in Rat Nonalcoholic Simple Fatty Liver [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(11): 2309-2317.
[3]	CHEN Miaomiao, TONG Xishuai, ZHENG Jiaming, ZHAO Hongyan, GU Jianhong, LIU Zongping. Effects of 5-amino-imidazole-4-carboxamide Nucleotide on Autophagy and Differentiation in Osteoclasts [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(11): 2339-2347.
[4]	LI Zhiqiang, YAN Shuping, JI Xiaoxia, WANG Kai, ZHANG Yuanshu. Distribution and Expression of Angiotensin-Converting Enzyme 2 (ACE2) in the Jejunum of Piglets and Its Relationship with Substance Transport [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(8): 1676-1684.
[5]	WEI Yu-shuang, LIU Jiao-yang, HAN Ying-qian, GUO Zhen-zhen, LIU Xiao-he, BA Gen, HAN Li-qiang, WANG Jiang, CHU Bei-bei, YANG Guo-yu. Construction of shRNA Library of Chlorocebus Sabaeus Cathepsin Genes and the Establishment of Its Stable Transfection Cell Lines [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(6): 1282-1290.
[6]	LI Long-long, PENG Meng-ling, WANG Dian, YU Lei, GE Chong-yang, MA Hai-tian. The Effect of (一)-Hydroxycitric Acid on Proliferation and Mitochondria Function in Cultured Primary Chicken Hepatocytes [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(5): 945-953.
[7]	NIE Pei-ting，TANG Cheng，YUE Hua. Cold-inducible RNA Binding Protein of Mice Involved in Response to Influenza A Virus Infection in Mice [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(8): 1652-1657.
[8]	QUAN Su-yu，ZHANG Yuan-shu，BU Deng-pan . Heat Stress-induced Cell Injury and Effects on the Gene Expression of Milk Synthesis-related Transporters in Dairy Cow [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(8): 1704-1713.
[9]	CHAI Ji-tian，JIANG Jun-na，LI Dong，WANG Jian-bing，ZHOU Jia-hua，LI Ying-ying，WANG Zhi-qiang. Analysis of CRE and SRE Response Element in the Porcine CCR1 and CCR5 Signal Pathway [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(9): 1650-1655.
[10]	GUO Bao-ping，XU Ming-long，HU Jin，XU Yin-xue. Studies on Changes of Sexual Organs and Their Related Hormones during the Early Phase in Dehydroepiandrosterone-induced Female Rat Polycystic Ovarian Syndrome Model [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(8): 1348-1355.
[11]	PAN Hong-bin，ZHAO Su-mei，SUN Ze-wei，LONG Guo-hui，GAO Shi-zheng，QIN Gui-xin. Fat-specific Protein 27 (Fsp27) and Lipid Metabolism [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(3): 347-353.
[12]	YANG Fan, TANG Cheng, YUE Hua. Interference of Chicken Heat Shock Protein 70 Gene Expression Induced ChickenEmbryo Fibroblast Apoptosis [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(3): 459-464.
[13]	HAN Haozhe, TIE Zihang, PANG Weijun, CAI Rui. Advances of IGF2BP2-Mediated m⁶A Modification on Animal Fat Deposition [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3605-3612.

Activation of Cold-inducible RNA-binding Protein by H1N1 Influenza Virus Contributes to Viral Replication Via Activating NF-κB Pathway

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 13

Recommended Articles

Metrics

Comments